Since 1912, the Jungfraujoch has been accessible to tourists by the Jungfrau line, a railway from Interlaken and Kleine Scheidegg, running partly underground through a tunnel through the Eiger and Mönch. The Jungfraujoch railway station, at an elevation of 3,454 metres (11,332 ft) is the highest in Europe. It lies east of the saddle, below the Sphinx station, and is connected to the Top of Europe building, which includes several panoramic restaurants, shops, exhibitions, and a post office. Several tunnels lead outside, where secured hiking trails on the crevassed glacier can be followed, in particular to the Mönchsjoch Hut. The normal route to the Jungfrau and Mönch starts from there.

The Sphinx Observatory, one of the highest astronomical observatories in the world, provides an additional viewing platform at a height of 3,572 metres (11,719 ft), the second-highest in Switzerland. It can be reached by an elevator from the Jungfraujoch. The observatory houses one of the Global Atmosphere Watch's atmospheric research stations.